RNA

Part:BBa_K199002

Designed by: Shamita Punjabi   Group: iGEM09_MoWestern_Davidson   (2009-06-26)

CCACU tRNA Suppressor (Produces Serine)

This gene encodes for a 5 base pair tRNA suppressor that suppresses CCACU, which would normally cause a frame shift mutation. It is used in conjuction with the Parts K199006 and K199005. Update: these tRNA parts do not work as expected, but instead appear to read four-base codons. See [http://www.ibc7.org/article/journal_v.php?sid=287]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 38
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aCCACU tRNA Suppressor (Produces Serine)